產品名稱：sAPP total ELISA 產 地：Demeditec 產品貨號：JP27731 產品規(guī)格：96 Tests 產品說明： Special remarks: For research use only, not for use in diagnostic procedures. INTRODUCTION Alzheimer's disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. It is known that Amyloid β (Aβ; which is major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. The production of soluble APPβ (sAPPβ) by β-secretase cleavage corresponds to Aβ production accordingly, so it is desired to measure sAPPβ in parallel with Aβ. In addition, it is reported that APP gene mutation exists in individuals who suffer early-onset familial Alzheimer’s disease. Swedish mutation, one of the APP gene mutations, is a double mutation at positions -1 to -2 from the β-secretase cleavage site (Lys670→Asn and Met671→Leu). And further, it is reported that Swedish mutation elevates Aβ40 and Aβ42 production (ref. 2), and that the mutation is utilized in establishment of transgenic mice (ref. 3). The measuring sAPPβ in Swedish type is useful for research of AD as well as in wild type. On the one hand, it is considered that in the metabolic pathway of APP, APP is first cleaved by α-secretase rather than β-secretase normally to produce soluble APPα (sAPPα) and subsequently P3 is cleaved from the remaining C-terminal fragment by γ-secretase. This kit can measure human soluble APP in samples as a total amount including sAPPα and sAPPβ. The recommended dilution for serum samples is more than 4-fold, for EDTA-plasma samples is more than 2-fold and for cerebrospinal fluids is more than 16-fold. PRINCIPLE This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the total quantities of human total sAPP. 上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數(shù)量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫(yī)學領域的應用。
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