產(chǎn)品名稱：IGFBP-3 mouse/rat ELISA 產(chǎn) 地：Demeditec 產(chǎn)品貨號：DEE031 產(chǎn)品規(guī)格：96 Tests 產(chǎn)品說明： Special remarks: Insulin-like growth factor binding protein 3, also known as IGFBP3, is a human gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Growth Hormone, Insulin-like Growth Factors and their binding proteins build up an endocrine system regulating not only longitudinal growth in humans but also influencing a broad variety of other physiological and pathophysiological processes like energy metabolism or tumor growth. Most effects of Growth Hormone (GH) are exerted by Insulin-like Growth Factors (IGF) mainly produced by the liver but also locally by specific tissues. The effects of IGF are also regulated, specific binding proteins (IGFBP 1-7) regulate bioavailability of IGF. After proteolytic cleavage of the binding proteins IGF is set free and able to bind to its receptor. The autophosphorylation of this thyrosine kinase receptor activates intra cellular signalling cascades. Some of these IGFBPs not only regulate the availability of IGF but also exert IGF-independent effects on cell physiology. IGFBP-3 ist the most abundant IGFBP in circulation and therefore of special relevance in regulation of IGF effects. This is reflected by the indicative value of serum IGFBP-3 concentration in diagnostics of growth disturbances. IGFBP-3 has also been shown to be able to induce apoptosis, promote tumor growth and inhibit cellular migration and metastasis dependent on tissue and tumor stage. Intended use of this ELISA testkit: This ELISA kit is suited for measuring IGFBP-3 in mouse and rat serum, cell lysates or nuclear extracts. Performance characteristics and validation of this testkit: This ELISA for mouse/rat IGFBP-3 (m/rIGFBP-3) is a so-called Sandwich-Assay. It utilizes two specific and high affinity antibodies for this protein. The IGFBP-3 in the sample binds to the immobilized first antibody on the microtiter plate. In the following step, the biotinylated and Streptavidin- Peroxidase conjugated second specific anti-mouse IGFBP-3-Antibody binds in turn to the immobilised mIGFBP-3. In the closing substrate reaction the turn of the colour will be high specific catalysed, quantitatively depending on the m/rIGFBP-3-level of the samples. The standards of this ELISA are recombinant mouse IGFBP-3 in concentrations of 0.078, 0.156, 0.313, 0.625, 1.25, 2.50 ng/ml and 5 ng/ml. Sensitivity The analytical sensitivity of this ELISA yields 0.018 ng/ml (2 SD of zero standard in 21fold determination). The Inter- and Intra-Assay variation coefficients were found less than 8.36 % and 4.36%. 上海玉博生物技術有限公司在為生命科學領域提供豐富的產(chǎn)品與信息資源方面處于****地位，公司提供的產(chǎn)品涵蓋了二十多個國家近五十萬種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長和更新，公司提供的產(chǎn)品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質(zhì)組學研究的認知，以及分子診斷和臨床醫(yī)學領域的應用。
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