CA 15-3 ELISA

產品名稱：CA 15-3 ELISA
產    地：Demeditec
產品貨號：DE5068
產品規格：96 Tests
產品說明：
Special remarks:
1 Introduction
1.1 Intended Use
The DEMEDITEC CA 15?3 ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of CA15?3 in serum and plasma.
1.2 Summary and Explanation
Breast cancer is one of the most common malignancies among women.  Nine categories of breast tumor showed evidence of clinical utility and were recommended for use in practice. Among these, CA 15-3 which detects soluble forms of MUC-1 protein is the most widely used serum marker in patients with breast cancer (1-6). The main use of CA 15-3 is for monitoring therapy in patients with metastatic disease (1, 11). Metastatic disease may be present at the time of initial diagnosis and can occur at any time following primary therapy. Up to 70% of patients with metastases will respond to systemic treatment with cytotoxic drugs or endocrine therapy; therefore, early detection of recurrence is important to patient management (7). In patients previously treated for stage II or stage III breast cancer, early detection of recurrence cannot be readily accomplished by routine clinical or diagnostic studies alone. The use of a circulating serum tumor marker assay, such as DEMEDITEC CA 15?3 ELISA, can be useful in the identification of these patients. In addition, CA 15-3 may also be used in the postoperative surveillance of asymptomatic women who have undergone surgery for invasive breast cancer (1,8). Finally, preoperative concentrations of CA 15-3 might be combined with existing prognostic factors for predicting outcome in patients with newly diagnosed breast cancer (9).
CA 15.3, although preferentially associated with breast cancer, is not tissue specific, and was shown to be elevated in different varieties such as ovarian cancer and colon adenocarcinoma (9). CA 15-3 assay values were not elevated in the sera of the majority of normal individuals or those with nonmalignant conditions (10, 11).
NOTE:  CA 15-3 values determined with different assays and from different manufacturers can vary due to differences in assay methods and reagent specificity. The results reported by the laboratory to the physician must include the identity of the assay used. Assay values obtained with different assay methods cannot be used interchangeably. In addition, the main limitation of CA 15-3 as a marker for breast cancer is that serum levels are rarely increased in patients with early or localized disease (1).
2 Principle of the test
The DEMEDITEC CA 15?3 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.
The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA15?3 molecule.
An aliquot of patient sample containing endogenous CA15?3 is incubated in the coated well with enzyme conjugate, which is an anti-CA15?3 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase is proportional to the concentration of CA15?3 in the sample.
Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA15?3 in the patient sample.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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