產品名稱：Estradiol ELISA sensitive 產 地：Demeditec 產品貨號：DE4399 產品規(guī)格：96 Tests 產品說明： Special remarks: Estradiol (1,3,5(10)-estratriene-3,17β-diol; 17β-estradiol; E21) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is affected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagine, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinising hormone (LH), which are essential for folicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiol levels have been reported to be increased in patients with feminising syndromes, gynaecomastia and testicular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment with, for example, clomiphene citrate, LH-releasing hormone (LH-RH), or exogenous gonadotropins. During ovarian hyperstimulation for in vitro fertislisation (IVF), serum estradiol concentrations are usually monitored daily for optimal timing of human chorionic gonadotropin (hCG) administration and oocyte collection. The above test kits measures the total (unconjugated) Estradiol in serum. There is also a method available for the measurement of free Estradiol in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DESLV4188 (please check the chapter "saliva testing"). This testkit coded DE4399 also can be used for veterinary investigations if the animal sample will be extracted using the following procedure: Extraction procedure for steroids We are recommending an extraction procedure as follows. 1.Pipette 1 ml of Ethyl Acetate in a 2.5 ml PP microtube (e.g. from Sarstedt). 2.Add between 5 and 200 µl of your sample. 3.Mix for 2-3 minutes on a vortexer. 4.Centrifuge the microtube briefly in order to achieve a clear separation of the 2 phases. 5.Deep freeze the microtube at -20°C or lower for several hours (or overnight). 6.Decant the organic liquid phase into a new tube which can be used for an evaporation process (the specification of the new tube depends of the specifications of your evaporating device). Be sure that no frozen material will be decanted. 7.Evaporate the organic solvent in an appropriate device at elevated temperature. The boiling point of Ethyl Acetate is 77°C. Please stay clearly below this temperature while evaporating. 8.After evaporating the organic solvent until complete dryness re-dissolve your sample with a diluent. In most cases this will be the zero standard of the ELISA (or RIA) to be used for the quantification of the analyte. 9.The volume of the diluent to be used for the reconstitution depends of the sensitivity of your assay system in relation to the analyte concentration in the primary sample. 10.The dilution (or concentration) factor depends of the volumes you have used in #1 and #7. 上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫(yī)學領域的應用。
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