Gastrin human I (1-17) ELISA

產品名稱：Gastrin human I (1-17) ELISA
產    地：Demeditec 
產品貨號：DE4187
產品規格：96 Tests
產品說明：
Special remarks:
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
1 DESCRIPTION
The DEMEDITEC human Gastrin I kit is a competitive immunoassay for the quantitative determination of Gastrin in samples. The kit uses a polyclonal antibody to Gastrin I to bind, in a competitive manner, the Gastrin in the sample or an alkaline phosphatase molecule which has Gastrin I covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Gastrin I in either standards or samples. The measured optical density is used to calculate the concentration of Gastrin I. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard or Tijssen.
2 INTRODUCTION
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are
associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Gastrin-I (G17) H2N-Glu-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2 Gastrin is synthesized as a 101 residue pre-pro-peptide on the rough endoplasmic reticulum, then
posttranslationally modified by cleavage and alpha-amidation to result in the active forms G34, G17 and G13/14; Big, Little and Mini-Gastrins respectively. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine residue, while
type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. In man, G17 has a circulating half-life of about 9 minutes while G34 has a half-life of about 35 minutes. G34 is the major circulating gastrin in fasting serum, but with G17, increases two to three-fold after feeding until both are present in approximately equal amounts. Fasting gastrin concentration is usually less than 30 pmol/L (equivalent to 62.9 pg/mL) while patients with Zollinger-Ellison Syndrome and
gastrinomas have levels exceeding 1,000 pg/mL.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

產品留言

標題

聯系人

聯系電話

內容

驗證碼

點擊換一張

注：1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!