hCG-b, free IRMA

產品名稱：hCG-b, free IRMA
產    地：Demeditec 
產品貨號：DE41100
產品規格：100 Tests
產品說明：
Special remarks:
Introduction
Human Chorionic Gonadotrophin (hCG) is a glycoprotein with a molecular weight of 38000, secreted by the trophoblast cells of placenta. It contains two different subunits. The a-subunit is common to all glycoprotein hormones and the ß-subunit is responsible for the immunological and biological specifity. Free form of both subunits are present in the circulation. Normal pregnancy is associated with an exponential increase of both holo-hCG and its free ß-subunit. hCG appears in the sera of pregnant women 5 days after the implantation of blastocyst and around the 10th week of pregnancy its concentration can reach up to 100 mIU/ml. The significant elevation of free ßhCG in maternal serum is now considered as a specific and sensitive diagnostic marker of gestational trophoblastic neoplasiae (GTN) including hydatidiform mole (both invasive and noninvasive) and choriocarcinoma. In addition to its use as a screening method, the appearence of free ßhCG after surgical abortion is a good indication of tumour recurrance. Free ßhCG has been proposed as one of the multi-parametric screening panel of Down syndrome, since this genetic disorder is associated with an extremely high concentration of free ßhCG of maternal serum in the first trimester. The ectopic production of free ßhCG is reported in a number of adenocarcinomas, in metastatic breast cancer and in testicular cancer.
Principle of method
The technology uses two monoclonal antibodies of high affinity in an immunoradiometric assay (IRMA) system. It offers an increased level of sensitivity and specificity compared with conventional RIA methods. This assay is based on a two-steps procedure to eliminate interference from serum protein. In the first stage the serum sample is incubated in streptavidin coated tubes with biotin labelled monoclonal antibody (capture). During an 1-hour incubation period with continuous agitation the immuno-complex is developed and immobilized on the reactive surface of test tubes. After incubation tubes are washed. In the second stage 125I labelled monoclonal antibody (signal) is added and it binds to an epitope of the free ßhCG molecule different from that recognised by the un­labelled capture-antibody developing the formation of a capture antibody - antigen - signal antibody complex, also referred to as a “sandwich”. Reaction mixture is then discarded, test tubes washed exhaustively, and the radioactivity is measured in a gamma counter. The concentration of antigen is directly proportional to the radioactivity measured in test tubes. By constructing a calibration curve plotting binding values against a series of calibrators containing known amount of free ßhCG, the unknown concentration of free ßhCG in patient samples can be determined.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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