Digoxin

產品名稱：Digoxin ELISA
產    地：Demeditec 
產品貨號：DE3268
產品規格：96 Tests
產品說明：
Special remarks:
SUMMARY AND EXPLANATION OF THE TEST
The clinical usefulness of the measurement of serum digoxin (DIG) is due to its low therapeutic ratio; a very small difference exists between therapeutic and toxic tissue levels.  In addition, individuals may vary in their response to digoxin with an apparent increase in susceptibility to toxicity with age. The action of digoxin is to increase the force and velocity of myocardial contraction.   This is necessary in the treatment of congestive heart failure and arrhythmias such as atrial fibrillation and atrial flutter. The myocardial concentrations of digoxin to serum levels remain relatively constant during normal renal function.  This distribution ratio of digoxin is approximately 29 to 1 between the heart and serum.  Thus, monitoring digoxin therapy by measurement of serum levels is feasible from the pharmacological standpoint, since serum levels are related to tissue levels following post-absorption equilibration.  A practical and sensitive method of digoxin quantitation in serum is by enzyme immunoassay.
This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations.  In this method, serum reference, patient specimen, or control is first added to a microplate well.  Digoxin coupled with enzyme horseradish peroxidase (HRP) is added, and the reactants are mixed. A competition reaction results between the enzyme conjugated digoxin and the native digoxin for a limited number of antibody combining sites immobilized on the well.
After the completion of the required incubation period, the antibody bound enzyme-digoxin conjugate is separated from the unbound enzyme-digoxin conjugate by aspiration or decantation.  Excess unbound enzyme reagent is washed via a wash step. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color.
The employment of several serum references of known digoxin concentration permits construction of a graph of activity and concentration.  From comparison to the dose response curve, an unknown specimen's activity can be correlated with digoxin concentration. The concentrations are expressed in ng/ml.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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