Androstenedione

產品名稱：Androstenedione ELISA
產    地：Demeditec 
產品貨號：DE3265
產品規格：96 Tests
產品說明：
Special remarks:
1 INTRODUCTION
1.1 Intended Use
The DEMEDITEC Androstenedione ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Androstenedione in serum and EDTA plasma
1.2 Summary and Explanation
The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. Testosterone, the most important biological active androgen, is derived from peripheral enzymatic conversion of Androstenedione.
In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary.
Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy.
In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or may be of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.
2 PRINCIPLE OF THE TEST
The DEMEDITEC Androstenedione ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with a polyclonal [rabbit] antibody directed towards an antigenic site of the Androstenedione molecule.  Endogenous Androstenedione of a patient sample competes with an Androstenedione-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase conjugate is inversely proportional to the concentration of Androstenedione in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Androstenedione in the patient sample.
There is also a method available for the measurement of free Androstenendione in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DESLV4780 (please check in the chapter "saliva testing".
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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