Vasopressin

產品名稱：Vasopressin RIA
產    地：Demeditec 
產品貨號：DE3021
產品規格：100 Tests
產品說明：
Special remarks:
1 Introduction
Vasopressin, or Antidiuretic Hormone (ADH) is a nine amino acid peptide which is cyclic in nature and has molecular weight of about 1060. Its structure is very similar to that of oxytocin, differing in only two amino acids. Endogenous ADH has antidiuretic and pressor activity, both approaching 400 units per mg, with an antidiuretic-to-vasopressin ration of 1, and a biphasic plasma half-life of 2.5 and 14.5 minutes. ADH is synthesised in the hypothalamic supraoptic nucleus and paraventricular nucleus of primates and transported via exonal flow to the posterior pituitary for storage and eventual release. The clinical application of a vasopressin radioimmunoassay is in diabetes insipidus, phychogenic water intoxication, hyponatraemia, stress conditions, ADH as a neurotransmitter and hypertension studies. ADH values can be influenced by cigarettes, tea, coffee, alcohol and some drugs.
2 Principle of the Test
After ethanol extraction of the plasma samples, Vasopressin is assayed by a competitive radioimmunoassay. Urinary Vasopressin can be measured directly. This radioimmunoassay is using a rabbit anti-Vasopressin antiserum and a radioiodinated Vasopressin [125I] tracer. Bound and free phases are separated by a second antibody bound to solid phase particles, followed by a centrifugation step. The radioactivity in the bound fractions is measured and a typical standard curve can be generated. The values of the extracted samples are corrected for extraction recovery.
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