產(chǎn)品名稱：AKT Activation Profile InstantOne™ ELISA 產(chǎn)品貨號：eBioscience 85-86045-11 產(chǎn)品規(guī)格：96 tests AKT Activation InstantOne™ ELISA Also known as: PKB, RAC-PK RUO: For Research Use Only. Not for use in diagnostic procedures. Attention: This 3 target InstantOne ELISAs contain reagents for a total of 96 wells (32 wells each for AKT (total), phospho-AKT (Thr308), and phospho-AKT (Ser473)). Please contact Technical Service for a lot specific COA SKU# 85-86045 Cat. No. Size 85-86045-11 96 tests Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total and phosphorylated AKT 1/2/3 (Thr308 and Ser473) in cell lysates. Detection of rat AKT 1/2/3 (Thr308 and Ser473) is also also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.
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