eFluor 514 Ca Sensor 1 mg

產品名稱：eFluor™ 514 Ca Sensor 1 mg
產品貨號：eBioscience 65-0859-39
產品規格：1 mg
Calcium Sensor Dye eFluor® 514
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 65-0859
Cat. No. Size
65-0859-39  1 mg 
65-0859-70  10 x 50 ug 
Description: eFluor™ 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ~520 nm . eFluor™ 514 Calcium Sensor Dye, like Fluo-3 and Fluo-4, is a commonly used dye among the visible light-excitable calcium indicators but with increased cellular uptake (even at room temperature) and brightness.
calcium binding affinity: Kd = 232 nM
Molecular Weight: approximately 1100
Peak Excitation: 490 nm
Peak Emission: 514 nm
eFluor™ 514 Calcium Sensor Dye should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 2-5 mM.
Once reconstituted, it should be protected from light and stored dessicated at -20°C; avoid freeze-thawing.

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