產品名稱：anti-rabbit TrueBlot™: Horseradish peroxidase (HRP) anti-rabbit IgG HRP 200 ul (1000X)
產品貨號：eBioscience 18-8816-33
產品規(guī)格：200 ul
Rabbit TrueBlot®: Anti-Rabbit IgG HRP
RUO: For Research Use Only. Not for use in diagnostic procedures.
SKU# 18-8816
Cat. No. Size
18-8816-31  50 uL 
18-8816-33  200 uL 
Description: Rabbit IgG TrueBlot® is a unique anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot, simply substitute the conventional anti-rabbit IgG blotting reagent with Rabbit IgG TrueBlot and follow the prescribed protocol for sample preparation and immunoblotting.
Note that there are three key procedural considerations:
1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-mouse IgG beads, anti-rat IgG beads, or anti-rabbit IgG beads should be used for mouse, rat, or rabbit immunoprecipitating antibodies, respectively.
2. Immunoprecipitate should be completely reduced.
3. Milk should be used as the blocking protein for the immunoblot.
 

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