細(xì)胞凋亡檢測(cè)試劑盒(FITC) In Situ Cell Death Detection Kit,Fluorescein
細(xì)胞凋亡檢測(cè)試劑盒(FITC)
羅氏細(xì)胞檢測(cè)系列產(chǎn)品自問世以來(lái)，歷經(jīng)時(shí)間的考驗(yàn)，以其**的品質(zhì)獲得了廣大業(yè)界用戶的支持和認(rèn)可，相關(guān)文獻(xiàn)達(dá)數(shù)千篇。
細(xì)胞凋亡產(chǎn)品特性：
☆檢測(cè)范圍廣泛：可實(shí)現(xiàn)對(duì)細(xì)胞培養(yǎng)液、組織切片、單個(gè)細(xì)胞及細(xì)胞群落的分析檢測(cè)，滿足日益增長(zhǎng)的高通量檢測(cè)需求。
☆檢測(cè)方法靈活：針對(duì)光學(xué)或熒光顯微鏡、流式細(xì)胞儀、ELISA。
☆操作**：無(wú)需接觸任何放射性同位素，簡(jiǎn)便易用。
☆產(chǎn)品線豐富：針對(duì)不同的細(xì)胞凋亡通路，有多種產(chǎn)品可供挑選。
一、檢測(cè)Caspase活性
①caspase對(duì)角蛋白18的裂解作用
產(chǎn)品名及貨號(hào)：M30 CytoDEATH 12140349001
M30 CytoDEATH,Fluorescein 12156857001
②Caspase-3活性
產(chǎn)品名及貨號(hào)：Caspase 3 Activity Assay 12012952001
③Caspase
產(chǎn)品名及貨號(hào)：Homogeneous Caspase Assay,fluorimetric 03005372001
④Caspase對(duì)RARP的裂解作用
產(chǎn)品名及貨號(hào)：Anti-Poly(ADP-Ribose)Polymerase(PARP) 11835238001
二、檢測(cè)DNA片斷
①TUNEL檢測(cè)法
產(chǎn)品名及貨號(hào)：In Situ Cell Death Detection Kit,AP 11684809910
In Situ Cell Death Detection Kit,POD 11684817910
In Situ Cell Death Detection Kit,Fluorescein 11684795910
In Situ Cell Death Detection Kit,TMR red 12156792910
②Elisa檢測(cè)
產(chǎn)品名及貨號(hào)：Cell Death Detection Elisa 11774425001
③凝膠電泳檢測(cè)
產(chǎn)品名及貨號(hào)：Apoptotic DNA Ladder Kit 11835246001
三、檢測(cè)膜變化
產(chǎn)品名及貨號(hào):Annexin-V-FLUOS 11828681001
Annexin-V-FLUOS Staining Kit 11988549001
Annexin-V-Alexa 568 03703126001
Annexin-V-Biotin 11828690001
四、檢測(cè)DNA合成
BrdU標(biāo)記檢測(cè)
產(chǎn)品名及貨號(hào)：Celluar DNA Fragmentation ELISA 11585045001
ROCHE相關(guān)產(chǎn)品資料
In Situ Cell Death Detection Kit, Fluorescein
Catalog :11684795910
Pack size :1 kit for up to 50 tests
Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology); analysis by fluorescence microscopy or flow cytometry.
Application:
Qualitative detection of apoptosis at the single-cell level by fluorescence microscopy and quantitative detection by flow cytometry.
Benefits:
- Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling
- Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction
- Convenient: No secondary detection system required
- Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis
Product Description:
Sample material: Cells in suspension, cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Background Information:
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2'-deoxy-uridine. The methods involve the separation of fragmented, low molecular weight DNA from unfragmented, high molecular weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population, or particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3'-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3'-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3'-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Contents
- Enzyme Solution (TdT), 5 vials
- Label Solution (fluorescein-dUTP), 5 vials
