Thiol reactive BDP FL maleimide是用于蛋白標(biāo)記的活性染料,它和BODIPY® FL maleimide有著完全相同的結(jié)構(gòu)。 BDP FL的吸收和發(fā)射光譜和熒光素(FAM)相近。然而,這種染料卻有著極好的耐光性,不帶電,分子量較低。它的亮度和熒光素、R110、呫噸染料衍生的如Alexa Fluor® 488。 這種熒光團很適用于熒光顯微鏡及其它應(yīng)用。該熒光團可以在很多應(yīng)用中替代熒光素,并適用于很多FAM兼容的儀器。
推薦手冊:
蛋白與其他硫醇分子的馬來酰亞胺標(biāo)記
BODIPY® is a trademark of Life Technologies. Alexa Fluor® is a trademark of Invitrogen.
General properties
Appearance:
orange solid
Mass spec M+ increment:
414.1
Molecular weight:
414.21
Molecular formula:
C20H21BF2N4O3
Solubility:
Good in organic solvents (DMF, DMSO), limited in water
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
503
Extinction coefficient at excitation maximum, Lmol-1cm-1:
80000
Emission maximum, nm:
509
Fluorescence quantum yield:
0.9
CF260:
0.015
CF280:
0.027
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